Determination of Tanshinones in Danshen (Salvia miltiorrhiza ) by High-Performance Liquid Chromatography with Fluorescence Detection after pre-Column Derivatisation

Determination of Astaxanthin in Trout by High Performance Liquid Chromatography Coupled with Visible Detection

Background: Astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout. Methods: A total of 20 trout samples were collected from local market and were analyzed for astaxanthin via HPLC-UV. Analyte extraction was done by water, methanol, dichloromethane, and acetonitrile 4.5:85:5:45.5, v/v/v/v. Results: Desp...

High Performance Liquid Chromatography Coupled with Pre-column Derivatization for Determination of Oxidized Glutathione Level in Rats Exposed to Paraquat

Glutathione (GSH) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ROS) which oxidizes to glutathione disulfide (GSSG). Paraquat (PQ), awidely used herbicide, causes pulmonary injury with the productionof ROS. Excessive ROS accumulation as a consequence of PQ exposure are frequently targeted by GSH thereby oxidative stress lead...

High Performance Liquid Chromatography Coupled with Pre-column Derivatization for Determination of Oxidized Glutathione Level in Rats Exposed to Paraquat

Glutathione (GSH) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ROS) which oxidizes to glutathione disulfide (GSSG). Paraquat (PQ), awidely used herbicide, causes pulmonary injury with the productionof ROS. Excessive ROS accumulation as a consequence of PQ exposure are frequently targeted by GSH thereby oxidative stress lead...

Determination of urinary norepinephrine and epinephrine by liquid chromatography with fluorescence detection and pre-column derivatization.

We assayed norepinephrine and epinephrine by utilizing solvent extraction, fluorescence derivatization, and "high-performance" liquid chromatography. A 100-microL aliquot of urine was extracted twice according to Smedes et al. (J Chromatogr 1982;231:25-39) and subsequently incubated with 1,2-diphenylethylenediamine. A 100-microL aliquot of the resulting mixture was injected into a reversed-phas...

Fluorescence Derivatisation in Liquid Chromatography